The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...

DIRECT azo dyes contain in most cases a number of coloured by-products which may adversely influence the shade of the dye, its stability in light and distort its biological action. In view of the ...

Most adsorption chromatography methods are based on a direct chemical interaction of a solid-phase ligand with a complementary target site on some population of biomolecules. Examples include ...

IAM consists of a monolayer of phosphatidylcholine covalently bound to an inert silica support. A highly reproducible method to measure drug adsorption, IAM chromatography is inexpensive and fast in ...