In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

Single-molecule localization microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image ...

Interfacial and near-surface processes are critical components for physical as well as biological systems, yet our understanding of these processes is limited by the lack of simple and robust imaging ...